Flow Cytometry and Cell SortingAndreas Radbruch Springer Science & Business Media, 29.06.2013 г. - 223 страници The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of these procedures, with emphasis on immunofluorescence (antibody-fluorochrome conjugation, staining principles and data evaluation); the isolation of specific chromosomes, cells and fragile, large particles by magnetic and fluorescence-activated sorting; cellular biochemistry; and the dynamics of proliferation. The methods have been field-tested in recent EMBO courses on flow cytometry. |
Съдържание
| 1 | |
Conjugation of Fluorochromes Haptens | 30 |
Multicolor Immunofluorescence Analysis | 47 |
Preparation and Staining of Cells | 64 |
Simultaneous Flow Cytometric Detection | 69 |
The Flow | 75 |
Indo1 Ratiometric Analysis | 87 |
Biochemical Parameters of Cell Function | 100 |
Powerful Preselection | 131 |
High Gradient Magnetic Cell Sorting | 141 |
Setup of a Flow Sorter | 153 |
Sorting of Rare Cells | 159 |
FluorescenceActivated Chromosome Sorting | 168 |
Flow Cytometry and Sorting of Plant Chromosomes | 177 |
Biological and Laser Safety | 207 |
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Често срещани думи и фрази
analyzed antibody antigen bandpass filter Becton Dickinson Biochem BrdU BrdU-Hoechst breakoff point buffer Ca2+ calcium calibration cell cycle cell sorting cell suspension cells/ml cellular cence centrifugation Chap chromomycin A3 chromosomes column concentration conjugates culture cytometer dead cells deflection detection droplet emission ethanol excitation FACS FACScan Ficoll FITC fixation flow cytometric analysis flow cytometry flow karyotype flow rate flow-in-air fluores fluorescein fluorescence microscope fluorochromes fraction frequency gating glutathione histograms Hoechst immunofluorescence increase Incubate indo-1 ratio intracellular karyotype kinetic labeling light longpass LRSC Tf lymphocytes MACS magnetic medium membrane metaphase method mg/ml microbeads microscope monocytes negative cells neutrophils nozzle number of cells optical optimal oxidative parameters particles PBS/BSA pellet phycobiliproteins phycoerythrin positive cells protein protocol protoplasts rare cells resuspend rhodamine room temperature sample tube separation sheath fluid Sigma signals solution specific stained cells staining reagents sterile surface tion washing wavelength µg/ml