Gene Transfer and Expression in Mammalian Cells, Том 38S.C. Makrides Gulf Professional Publishing, 24.10.2003 г. - 680 страници The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field. |
Съдържание
Stable gene expression | 14 |
Using HSV1 to make gene therapy vectors | 33 |
Applications of HSV vectors in the nervous system | 42 |
Applications outside the nervous system | 48 |
Cellspecific gene expression | 64 |
Abbreviations | 89 |
Production of recombinant AAV vectors | 97 |
In vitro and in vivo gene transfer | 99 |
Conclusions | 165 |
Poliovirus vector development and its immunogenic potential | 176 |
Abbreviations | 185 |
Insect and crustacea | 195 |
Abbreviations | 203 |
Expression of topologically different proteins | 209 |
Scaleup of protein production | 215 |
Expression in primary cell cultures | 216 |
Abbreviations | 105 |
Gene delivery | 115 |
Production and analyses of viral particles | 121 |
Construction of recombinant poxvirus vectors | 127 |
Laboratory and clinical applications | 132 |
Molecular biology of virus vector construction | 139 |
Applications of the baculovirus mammalian gene delivery vector | 145 |
Abbreviations | 226 |
Development of recombinant retrovirus vectors | 235 |
Expression of the transgene | 242 |
References | 248 |
HIV1derived vector packaging system | 255 |
Gene transfer applications | 262 |
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