Flow Cytometry and Cell SortingAndreas Radbruch Springer-Verlag, 1992 - 223 страници Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced. In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible. |
Съдържание
Introduction L A Herzenberg | 1 |
Conjugation of Fluorochromes Haptens | 27 |
Multicolor Immunofluorescence Analysis | 47 |
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activation analyzed antibody antigen Becton Dickinson Biochem BrdU BrdU-Hoechst breakoff point buffer Ca2+ calcium calibration cell cycle cell sorting cell suspension cellular cence centrifugation Chap chromomycin A3 chromosomes column concentration conjugates culture cytometer dead cells deflection detection droplet emission ethanol excitation FACS FACScan Ficoll FITC fixation flow cytometric analysis flow cytometry flow karyotype flow rate flow-in-air fluores fluorescein fluorescence microscope fluorochromes fMLP fraction frequency gating glutathione green fluorescence histograms Hoechst immunofluorescence increase Incubate indo-1 ratio intracellular karyotype kinetic labeling light longpass LRSC Tf lymphocytes MACS magnetic membrane metaphase method mg/ml microbeads microscope monocytes NaN3 negative cells neutrophils nozzle number of cells optical optimal oxidative parameters particles PBS/BSA pellet phycobiliproteins phycoerythrin positive cells protein protocol protoplasts rare cells resuspend rhodamine room temperature sample tube separation sheath fluid Sigma signals solution specific stained cells sterile surface tion Troubleshooting washing wavelength µg/ml